Ransfection assays [11]. To determine if these proteins, together with BORIS, can form secondary complexes with each other in the absence of DNA and to understand better the mechanism underlying the regulation of MAGE-A1 expression, we carried out in vitro proteinprotein interaction assays. Each of the proteins was either a resin-bound "bait" fusion protein or a [35S]-L-methionine labeled "prey" p
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