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IcsResultsAssociations of DC-SCRIPT with clinicopathological factors and histological and intrinsic breast cancer subtypesThe relationship between DC-SCRIPT and patient and tumor characteristics was investigated with the use of non-parametric methods (Spearman rank correlations for continuous variables and Wilcoxon rank-sum for dichotomized or Kruskal-Wallis test for ordered variables). To reduce
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Ransfection assays [11]. To determine if these proteins, together with BORIS, can form secondary complexes with each other in the absence of DNA and to understand better the mechanism underlying the regulation of MAGE-A1 expression, we carried out in vitro proteinprotein interaction assays. Each of the proteins was either a resin-bound "bait" fusion protein or a [35S]-L-methionine labeled "prey" p
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S (B).Figure 7B schematically summarizes the protein-protein interactions.Discussion In the current study devoted mainly to understanding the transcriptional regulation of MAGE-A1, we detected that ectopically expressed BORIS was able to induce MAGE-A1 promoter activity in MCF-7 cells and micrometastatic BCM1 cells. This activation by BORIS was associated with DNA demethylation of the MAGE-A1 prom
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S (B).Figure 7B schematically summarizes the protein-protein interactions.Discussion In the current study devoted mainly to understanding the transcriptional regulation of MAGE-A1, we detected that ectopically expressed BORIS was able to induce MAGE-A1 promoter activity in MCF-7 cells and micrometastatic BCM1 cells. This activation by BORIS was associated with DNA demethylation of the MAGE-A1 prom
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